Presbyterian worship began in Adelaide in 1839. Chalmers Church was built in 1851 by a group of Scottish families. It was originally named Chalmers Cghurch to honour the Reverend Thomas Chalmers who led the ‘Disruption’ in Scotland, which resulted in the birth of the Free Church of Scotland. In 1929, local Presbyterians decided to form a united parish under the joint name of Scots Church – the present name of the church. The foundation stone of the church was never found. In 1858 the spire and bell were added, and in 1864 a two-level schoolroom was built at the rear of the church. Later a Church hall and offices were added.

There are three fairly recent stained glass windows in the front north wall named The Celebration of Creation designed in 1962. The church contains a pipe organ, an organ screen and Iona Cross. There is also a massive oak communion table and a carved wooden pulpit featuring the symbol of the burning bush. The motto ‘Burning yet not consumed’ around the burning bush represents the church's belief that God’s love revealed in Christ, continues to be offered to people in every age and place.

The sanctuary is open to the public at certain times during the week.

Text largely taken from the ‘Information for Visitors’ pamphlet.